35. Solving a chemistry problem

35. Solving a chemistry problem

63. For the fluorimetric determination of riboflavin, 5 cm3 of the analyzed solution was transferred to a volumetric flask with a capacity of 250 cm3; The volume of the solution was brought to the mark with purified water. The fluorescence intensity of the resulting solution, the standard solution (0.5 μg/cm3) and the reference solution was measured using a fluorimeter. The device readings are respectively 42.50; 80; 0.10. Calculate the mass (in grams) of riboflavin in 100 cm3 of the analyzed solution. M.r. (C17H20N4O6)=376.37.

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