47. Solving a chemistry problem

47. Solving a chemistry problem

312. The boundary of sedimentation (the boundary between the cloudy and transparent parts of the solution) of serum globulin in phosphate buffer solution at 26 C is measured as a function of time at a centrifuge speed of 44,000 min–1:

t, min 30 50 70 90 110 130 150 170
x*, cm 4.72 4.79 4.86 4.93 4.99 5.06 5.13 5.21
* x - distance from the axis of rotation
Based on these data, determine the sedimentation coefficient, as well as the molar mass of the protein, knowing that the diffusion coefficient is 5.27 × 10–7 cm2/s, the specific partial volume of the protein is 0.745 cm3/g, and the density of the buffer solution is 1.013 g/cm3.

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