94. Solving a chemistry problem

94. Solving a chemistry problem

56. The optical density of 1.00 * 10–4 mol / l of a solution of a certain substance was measured at various wavelengths in a cuvette with an absorbing layer thickness of 5.00 cm (option a), 1.00 cm (options b, d, e), 0.50 cm (option c). Using the data below, construct the absorption spectrum of the test solution in the coordinates optical density A - wavelength λ, nm.
Select the analytical wavelength at which the greatest accuracy and sensitivity of determining the substance by the spectrophotometric method is achieved, and at the selected wavelength, calculate the molar extinction coefficient of this substance in solution.

Wavelength, nm Optical density
Option
b
350 0.784
355-
360 0.392
365 0.366
370 0.376
380 0.455
390 0.523
395 0.540
400 0.537
410 0.454
420 0.284
430 0.193
435-
440 0.183
450 0.157
460 0.088
470 0.044
480 0.018
490 0.009
500 0.003

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